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Characterization of Metabolites in an Endophytic Fungus Diaporthe fraxini via NMR-based Metabolomics and Cholinesterase Inhibitory Activity

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Abstract

Endophytic Diaporthe is a fungal genus having an extensive distribution in plant hosts. It is known as a valuable source of bioactive metabolites with potent biological properties. In the present study, proton nuclear magnetic resonance (1H-NMR) coupled with multivariate analysis (MVA) was employed to discriminate the chemical profile of Diaporthe fraxini cultured under different growth conditions. Cholinesterase inhibitory assay was performed to assess the activity of the fungal extracts against acetylcholinesterase and butyrylcholinesterase. Discriminant metabolites responsible for the chemical variations were successfully obtained using 1H-NMR-based metabolomics approach. Principal component analysis showed a clear discrimination of the fungal extracts of D. fraxini grown under different conditions. Cholinesterase inhibitory activity studies revealed the potential of supplemented cultured fungal extract of D. fraxini as a source of cholinesterase inhibitor.

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ACKNOWLEDGMENTS

The authors would like to acknowledge Ministry of Higher Education Malaysia for Fundamental Research Grant Scheme with Project Code: FRGS/1/2018/STG01/USM/02/3.

Funding

This study was funded by the Ministry of Higher Education Malaysia for Fundamental Research Grant Scheme with Project Code: FRGS/1/2018/STG01/USM/02/3.

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Authors and Affiliations

  1. Chemistry Section, School of Distance Education, University Sains Malaysia, 11800, Penang, Malaysia

    K. Nagarajan & W.-N. Tan

  2. School of Pharmaceutical Sciences, University Sains Malaysia, 11800, Penang, Malaysia

    B. Ibrahim & A.A. Bawadikji

  3. Department of Clinical Pharmacy & Pharmacy Practice, Faculty of Pharmacy, University Malaya, 50603, Kuala Lumpur, Malaysia

    B. Ibrahim

  4. School of Pharmacy, Monash University Malaysia, 47500, Selangor, Malaysia

    K.-Y. Khaw

  5. University Kuala Lumpur, Branch Campus Institute of Medical Science Technology (MESTECH), 43000, Selangor, Malaysia

    W.-Y. Tong

  6. University Kuala Lumpur, Branch Campus Malaysian Institute of Chemical and Bioengineering Technology (MICET), 78000, Melaka, Malaysia

    C.-R. Leong

  7. Centre for Drug Research, University Sains Malaysia, 11800, Penang, Malaysia

    S. Ramanathan

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  1. K. Nagarajan
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Correspondence to W.-N. Tan.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Nagarajan, K., Ibrahim, B., Bawadikji, A. et al. Characterization of Metabolites in an Endophytic Fungus Diaporthe fraxini via NMR-based Metabolomics and Cholinesterase Inhibitory Activity. Appl Biochem Microbiol 59, 316–322 (2023). https://doi.org/10.1134/S0003683823030134

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  • DOI: https://doi.org/10.1134/S0003683823030134

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