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Cadaverine Biosynthesis in Escherichia сoli Adaptation to Hydrogen Peroxide

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Abstract

This study demonstrated the role of cadaverine in the adaptation of Escherichia coli to hydrogen peroxide. A dose-dependent increase in the expression of the ldcC and cadA genes that encode cadaverine-synthesizing enzymes was shown in cells exposed to exogenous hydrogen peroxide. An inverse relationship between bacterial sensitivity and the level of intracellular cadaverine at the time of hydrogen peroxide addition was found. Sensitivity to hydrogen peroxide also depended on the ability to synthesize cadaverine (via the ldcC and cadA genes). In particular, the death rate of cells that are incapable of synthesizing cadaverine was higher compared to cadaverine-producing cells. Sensitivity to hydrogen peroxide increased in a series of isogenic strains with the genotype: wild type < ΔcadA < ΔldcC < ΔldcCΔcadA. The role of the accumulation of reactive oxygen species, including hydrogen peroxide, in the activation of the ldcC and cadA genes in cells exposed to the antibiotic levofloxacin was also shown.

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ACKNOWLEDGMENTS

The authors express their sincere gratitude to Professor Bruce Demple (Stony Brook University Medical School, Stony Brook, NY) for providing the E. coli strain.

Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation (АААА-А19-119112290009-1).

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 614081, Perm, Russia

    A. V. Akhova & A. G. Tkachenko

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  1. A. V. Akhova
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  2. A. G. Tkachenko
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Correspondence to A. V. Akhova.

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The authors declare they have no conflicts of interest. The studies were conducted without the use of animals and without involving people as subjects.

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Akhova, A.V., Tkachenko, A.G. Cadaverine Biosynthesis in Escherichia сoli Adaptation to Hydrogen Peroxide. Appl Biochem Microbiol 58, 582–589 (2022). https://doi.org/10.1134/S0003683822050039

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