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Conversion of phytosterols into androstenedione by Mycobacterium neoaurum

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Abstract

Optimum conditions for transformation of phytosterols by Mycobacterium neoaurum, required for selective cleavage of the lateral chain into androstenedione, were shown to differ from the known conditions of animal sterol (cholesterol) transformation. Complete conversion of phytosterols into androstenedione at a substrate load of no less than 20 g/l was achieved on increasing the amount of the inoculum and the concentration of glucose (by 2 and 4 times, respectively, relative to cholesterol) and performing the fermentation under conditions of turbulent mixing. Under these conditions, both the rate of the transformation and the yield of the reaction product were high, due to the saturation of the culture liquid with hydrocarbonate. Data from the literature show that this ion is involved in cleavage of the branched lateral chain at carbon in position 24.

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Authors and Affiliations

  1. Bioengineering Center, Russian Academy of Sciences, Moscow, 117312, Russia

    N. V. Rodina, M. A. Molchanova, N. E. Voishvillo, V. A. Andryushina & T. S. Stytsenko

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  1. N. V. Rodina
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  2. M. A. Molchanova
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  3. N. E. Voishvillo
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  4. V. A. Andryushina
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  5. T. S. Stytsenko
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Corresponding author

Correspondence to V. A. Andryushina.

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Original Russian Text © N.V. Rodina, M.A. Molchanova, N.E. Voishvillo, V.A. Andryushina, T.S. Stytsenko, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 1, pp. 56–62.

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Rodina, N.V., Molchanova, M.A., Voishvillo, N.E. et al. Conversion of phytosterols into androstenedione by Mycobacterium neoaurum . Appl Biochem Microbiol 44, 48–54 (2008). https://doi.org/10.1134/S0003683808010080

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  • DOI: https://doi.org/10.1134/S0003683808010080

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