Abstract
Pannexin1 and pannexin2 are members of the pannexin gene family which are widely expressed in the central nervous system. Here we present an overview of pannexin expression and distribution in the mouse cerebellum. Pannexin1 and pannexin2 are expressed in the Purkinje cells and in some cells of the granule cell layer. Pannexin2 is also expressed in the stellate cells of the molecular layer. A differential expression of pannexin1 and pannexin2 mRNA is observed during cerebellar development. These findings constitute the first indication of the involvement of pannexin molecules in the develo** cerebellum. Although the functional relevance of these molecules remains currently unknown, the abundance of pannexins in the Purkinje cells suggests that they may contribute to the generation of cerebellar rhythms.
Similar content being viewed by others
References
Phelan P. Innexins: Members of an evolutionarily conserved family of gap-junction proteins. Biochim Biophys Acta. 2005;1711:225–245.
Phelan P, Bacon JP, Davies JA, et al. Innexins: A family of invertebrate gap-junction proteins. Trends Genet. 1998;14: 348–349.
Panchin Y, Kelmanson I, Matz M, Lukyanov K, Usman N, Lukyanov S. A ubiquitous family of putative gap junction molecules. Curr Biol. 2000;10:R473–474.
Vogt A, Hormuzdi SG, Monyer H. Pannexin1 and Pannexin2 expression in the develo** and mature rat brain. Brain Res Mol Brain Res. 2005;141:113–120.
Ray A, Zoidl G, Weickert S, Wahle P, Dermietzel R. Sitespecific and developmental expression of pannexin1 in the mouse nervous system. Eur J Neurosci. 2005;21:3277–3290.
Bruzzone R, Hormuzdi SG, Barbe MT, Herb A, Monyer H. Pannexins, a family of gap junction proteins expressed in brain. Proc Natl Acad Sci USA. 2003; 100:13644–13649.
Baranova A, Ivanov D, Petrash N, et al. The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins. Genomics. 2004;83:706–716.
Panchin YV. Evolution of gap junction proteins — the pannexin alternative. J Exp Biol. 2005;208:1415–1419.
Weickert S, Ray A, Zoidl G, Dermietzel R. Expression of neural connexins and pannexin1 in the hippocampus and inferior olive: A quantitative approach. Brain Res Mol Brain Res. 2005;133:102–109.
Bruzzone R, Barbe MT, Jakob NJ, Monyer H. Pharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes. J Neurochem. 2005;92:1033–1043.
Dusart I, Airaksinen MS, Sotelo C. Purkinje cell survival and axonal regeneration are age dependent: Anin vitro study. J Neurosci. 1997;17:3710–3726.
Garcia-Segura LM, Baetans D, Roth J, Norman AW, Orci L. Immunohistochemical map** of calcium-binding protein immunoreactivity in the rat central nervous system. Brain Res. 1984;296:75–86.
Rogers JH. Immunoreactivity for calretinin and other calcium-binding proteins in cerebellum. Neuroscience. 1989;31:711–721.
Celio MR. Calbindin D-28k and parvalbumin in the rat nervous system. Neuroscience. 1990;35:375–475.
Kutzleb C, Sanders G, Yamamoto R, et al. Paralemmin, a prenyl-palmitoyl-anchored phosphoprotein abundant in neurons and implicated in plasma membrane dynamics and cell process formation. J Cell Biol. 1998;143:795–813.
Montoro RJ, Yuste R. Gap junctions in develo** neocortex: A review. Brain Res Brain Res Rev. 2004;47:216–226.
Dermietzel R, Traub O, Hwang TK, et al. Differential expression of three gap junction proteins in develo** and mature brain tissues. Proc Natl Acad Sci USA. 1989;86: 10148–10152.
Kandler K, Katz LC. Neuronal coupling and uncoupling in the develo** nervous system. Curr Opin Neurobiol. 1995;5:98–105.
Martinez S, Geijo E, Sanchez-Vives MV, Puelles L, Gallego R. Reduced junctional permeability at interrhombomeric boundaries. Development. 1992;116:1069–1076.
Melloy PG, Kusnierczyk MK, Meyer RA, Lo CW, Desmond ME. Overexpression of connexin43 alters the mutant phenotype of midgestational wnt-1 null mice resulting in recovery of the midbrain and cerebellum. Anat Rec A Discov Mol Cell Evol Biol. 2005;283:224–238.
Crochet S, Fuentealba P, Timofeev I, Steriade M. Selective amplification of neocortical neuronal output by fast prepotentialsin vivo. Cereb Cortex. 2004;14:1110–1121.
Grenier F, Timofeev I, Steriade M. Focal synchronization of ripples (80–200 Hz) in neocortex and their neuronal correlates. J Neurophysiol. 2001;86:1884–1898.
Draguhn A, Traub RD, Schmitz D, Jefferys JG. Electrical coupling underlies high-frequency oscillations in the hippocampusin vitro. Nature. 1998;394:189–192.
Schmitz D, Schuchmann S, Fisahn A. Axo-axonal coupling. A novel mechanism for ultrafast neuronal communication. Neuron. 2001;31:831–840.
Traub RD, Bibbig A, Fisahn A, LeBeau FE, Whittington MA, Buhl EH. A model of gamma-frequency network oscillations induced in the rat CA3 region by carbachol in vitro. Eur J Neurosci. 2000;12:4093–4106.
Cheron G, Gall D, Servais L, Dan B, Maex R, Schiffmann SN. Inactivation of calcium-binding protein genes induces 160 Hz oscillations in the cerebellar cortex of alert mice. J Neurosci. 2004;24:434–441.
Bennett MV, Zukin RS. Electrical coupling and neuronal synchronization in the mammalian brain. Neuron. 2004;41: 495–511.
Bao L, Locovei S, Dahl G. Pannexin membrane channels are mechanosensitive conduits for ATP. FEBS Lett. 2004;572: 65–68.
Stout CE, Costantin JL, Naus CC, Charles AC. Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels. J Biol Chem. 2002;277:10482–1048.
Bennett M. V. Contreras JE, Bukauskas FF, Saez JC. New roles for astrocytes: Gap junction hemichannels have something to communicate. Trends Neurosci. 2003;26:610–617.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ray, A., Zoidl, G., Wahle, P. et al. Pannexin expression in the cerebellum. Cerebellum 5, 189–192 (2006). https://doi.org/10.1080/14734220500530082
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1080/14734220500530082