Summary
An efficient and rapid in vitro method was developed for regeneration of Phalaenopsis using leaf segments derived in vitro from flower stalk nodes. Leaf segments of four cultivars Tinny Sunshine ‘Annie’, ‘Taisuco Hatarot’, Teipei Gold ‘Golden Star’, Tinny Galaxy ‘Annie’ cultured on Murashige and Skoog medium supplemented with N 6-benzyladenine (BA; 88,8 μM) and α-naphthaleneacetic acid (NAA; 5,4 μM) produced an average of 10–13 protocorm-like bodies (PLBs) after 12 wk. PLB proliferation was achieved on a modified Hyponex medium (1 gl−1 6.5N−4.5P−19K+20N−20P−20K+2gl−1 peplone +3% (w/v) potato homogenate +0.05% activated 1 gl−1 charcoal) and an optimal number of 13–18 PLBs developed from single PLB sections of different cultivars. Plantlet development was also achieved on a modified Hyponex medium. By repeated subculture of PLBs on a proliferation medium, and culturing them in the plantlet regeneration medium, plantlets could be produced continuously. Approximately 6 mo, were required from the initiation of culture to the production of plantlets for transplant to community pots.
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References
Arditti, J.; Earnst, R. Micropropagation of orchids. New York: Wiley: 1993:467–520.
Chen, Y. C.; Chang, C.; Chang, W. C. A reliable protocol for plant regeneration from culture of Phalaenopsis. In vitro Cell. Dev. Biol. Plant 36:420–423; 2000.
Haas-von Schmude, N. F. Klonale Massenvemehrung, von Phalaenopsis. Die Orchidee 34:242–248; 1983.
Haas-von Schmude, N. F. Tissue culturing Phalaenopsis using leaves and leaf segments. In: Tan, K., ed. Proc. 11th Orchid Conference. Miami; 1985:311.
Kano, K. Studies on the media for orchid seed germination. Mem. Fac. Agri. Kagawa Univ. 20:1–68: 1965.
Knudson, L. A new nutrient solution for germination of orchid seed. Am. Orchid Soc. Bull. 15:214–217; 1946.
Lakshmanan, P.; Loh, C. S.; Goh, C. J. An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture. Plant Cell Rep. 14:510–514; 1995.
Lindemann, E. G. P.; Gunckel, J. E.; Davidson, O. W. Meristem culture of Cattleya. Am. Orchid Soc. Bull. 39:100–127; 1970.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Park, S. Y.; Murthy, H. N.; Paek, K. Y. Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss. Organ Cult. 63:67–72; 2000.
Reuter, E. The importance of propagating Phalaenopsis by tissue culture. Orchid Rev. 91:199–201; 1983.
SAS Institute. SAS/STAT user's guide, 4th edn, version 6, Cary, NC: SAS Institute; 1989.
Tanaka, M. Studies on the clonal propagation of Phalaenopsis through in vitro culture. Mem. Fac. Agric. Kagawa Univ. 49:1–85; 1987.
Tanaka, M. Micropropagation of Phalaenopsis spp. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, vol. 20, Berlin: Springer-Verlag 1992:246–268.
Tanaka, M.; Sakanishi, Y. Clonal propagation of Phalaenopsis by leaf culture. Am. Orchid Soc. Bull. 46:733–737; 1977.
Tanaka, M.; Sakanishi, Y. Clonal propagation of Phalaenopsis through tissue culture. In: Kashemsanta, M. R. S., ed. Proc. 9th World Orchid Conference, Bangkok; 1980:215–221.
Tanaka, M.; Sakanishi, Y. Regenerative capacity of in vitro cultured leaf segments excised from mature Phalaenopsis plants. Bull. Univ. Osaka Prefect Ser. B. 37:1–4; 1985.
Vacin, E.; Went F. Some pH changes in nutrient solutions. Bot. Gaz. 110:605–613; 1949.
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Park, SY., Murthy, H.N. & Paek, KY. Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In Vitro Cell.Dev.Biol.-Plant 38, 168–172 (2002). https://doi.org/10.1079/IVP2001274
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DOI: https://doi.org/10.1079/IVP2001274