Extended Data Fig. 2: Ex-vivo stimulated healthy PBMCs were stained with a panel of antibodies and analyzed by CyTOF and Luminex.

A. Expression of surface canonical markers using viSNE in Cytobank. The distribution of each of the clustering parameters is presented as a color scale in z-dimension for the manual identification of each cluster immune population in Fig. 2. B. Expression of surface canonical markers visualized using a heatmap. C. Batch correction of CyTOF data from 2 independent experiments used for the results of Fig. 2. Blue dots correspond to responses to healthy plasma (n = 10 biologically independent samples), red dots to atherosclerosis plasma (n = 20 biologically independent samples; males=10). D. Dot plots show the effect of atherosclerotic plasma (n = 20 biologically independent samples; males=10) vs. healthy plasma (n = 10 biologically independent samples) on the phosphorylation of intracellular kinases in CD14⁺ monocytes and CD1c⁺ DCs. P values were determined by unpaired two-tailed t-test. Data are presented as mean values +/- SD. E. Dot plots show the effect of atherosclerotic plasma (n = 20 biologically independent samples; males=10) vs. healthy plasma (n = 10 biologically independent samples) on the phosphorylation of intracellular kinases in CD16⁺ monocytes. P values were determined by unpaired t-test, two-tailed. Data are presented as mean values +/- SD. F. Heatmap of cytokine levels in plasma of atherosclerotic (red, n = 20 biologically independent samples; males=10) vs. healthy donors’ (blue, n = 15 biologically independent samples) plasma with clustering based on standardized z-scores of cytokine values and correspondent concentrations (pg/ml). G. Point plot of cytokines levels in plasma of PBMCs stimulated with atherosclerotic patient plasma (n = 20 biologically independent samples; males=10) vs healthy plasma (n = 15 biologically independent samples). P values were determined by unpaired two-tailed t-test.