Extended Data Fig. 2: Cardiac-specific TGR5 deletion aggravates cardiac dysfunction and cardiolipotoxicity in female mice with HFD/STZ-induced diabetic cardiomyopathy.
From: Inhibition of fatty acid uptake by TGR5 prevents diabetic cardiomyopathy
a, Left ventricular global longitudinal strain (GLS) was calculated using Vevo Software. n = 6. b, Ratio of flow Doppler E wave amplitude to tissue Doppler E’ wave amplitude (E/E’). n = 6. c, Representative left ventricular M-mode echocardiographic images. d, Quantification of left ventricular ejection fraction (EF). n = 6. e, Quantification of left ventricular fractional shortening (FS). n = 6. f, Representative images of H&E, WGA, Masson’s trichrome, Oil Red O and BODIPY 493/503 staining (green color) images of cardiac tissues from TGR5fl/fl and TGR5ΔCM mice challenged with HFD/STZ. Scale bar, 2.5 mm for H&E; 10 μm for WGA; 2.5 mm and 100 μm for Masson’s trichrome staining; 50 μm for Oil Red O staining; 10 μm for BODIPY 493/503 staining. The nucleus was stained with DAPI (blue color). g, Quantification of myocyte area in WGA staining. n = 6. h, Quantification of cardiac fibrosis area in Masson’s trichrome staining. n = 6. i, Quantitative analysis of Oil Red O staining. n = 6. j, BODIPY intensity by Image J. n = 6. Data are presented as mean ± SEM. Statistical significance was evaluated by two-tailed nonparametric Mann–Whitney test (i,j), or two-way ANOVA followed by Tukey’s post-hoc test (a,b,d,e,g,h).