Extended Data Fig. 4: DCA and TCA prevent against cardiac dysfunction and cardiolipotoxicity in mice with HFD/STZ-induced diabetic cardiomyopathy.
From: Inhibition of fatty acid uptake by TGR5 prevents diabetic cardiomyopathy
a–d, TGR5fl/fl and TGR5ΔCM mice challenged with HFD/STZ were treated with DCA or TCA for 12 weeks. Left ventricular GLS was calculated by Vevo Software (a). n = 6. Ratio of flow Doppler E wave amplitude to tissue Doppler E′ wave amplitude (E/E’) (b). n = 6. Quantification of left ventricular EF (c) and FS (d). n = 6. e, Representative images of H&E, WGA, Masson’s trichrome, Oil Red O and BODIPY 493/503 staining (green color) images of cardiac tissues from TGR5fl/fl and TGR5ΔCM mice with DCA, TCA or vehicle treatment for 12 weeks. Scale bar, 2.5 mm for H&E; 10 μm for WGA; 2.5 mm and 100 μm for Masson’s trichrome staining; 50 μm for Oil Red O staining; 10 μm for BODIPY 493/503 staining. The nucleus was stained with DAPI (blue color). f, Quantification of myocyte area in WGA staining. n = 6. g, Quantification of cardiac fibrosis area in Masson’s trichrome staining. n = 6. h, Quantitative analysis of Oil Red O staining. n = 6. i, BODIPY intensity by Image J. n = 6. Data are presented as mean ± SEM. Statistical significance was evaluated by one-way ANOVA followed by Tukey’s post-hoc test.