Extended Data Fig. 2: Genetic silencing of c-MYC impairs osteoclastogenesis and serine synthesis pathway-related gene expression.
a cMyc, Nfatc1, Phgdh, Psat1 mRNA levels in osteoclasts after transduction with a lentiviral vector carrying a shRNA against c-MYC (shMYC) (n = 4; unpaired, two-tailed Student’s t-test). An empty vector was used as control. b, c TRAP staining (b) and quantification of control and c-MYC-silenced TRAP+ multinuclear cells (MNCs; c) (n = 4 biologically independent samples; unpaired, two-tailed Student’s t-test). Scale bar is 50 µm. Data are means ± s.d.