Extended Data Fig. 6: NAD+ precursors (NMN or NR) treatment restored mitochondria respiration capacity, lowered apoptosis, and improved energy production.
From: NAD+ precursor supplementation prevents mtRNA/RIG-I-dependent inflammation during kidney injury
a, Cytotoxicity assay. The data is as represented as fold change (FC) normalized to control PBS group (n = 8 in each group). b, Relative transcript levels of Bax in renal tubule cells of experimental groups (n = 3 in each group). Gene expression levels were normalized to Gapdh. c, The result of oxygen consumption rate (OCR) in cultured renal tubule cells of experimental groups PBS n = 6. Cis + PBS n = 6. Cis + NMN n = 5. Cis + NR n = 5. *P < 0.05. The data was normalized to total protein levels. d, ATP levels in renal tubule cells in experimental groups (n = 3 in each group). *P < 0.05. The data was normalized to total protein levels. e, Live cell numbers of cisplatin-treated renal tubule cells in indicated experiment groups (n = 3 in each group). veh; vehicle control. NS, not significant. Data are presented as mean ± s.e.m. and were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison.