Extended Data Fig. 2: Doxycycline promotes cell survival through attenuation of mitochondrial translation.
From: Tetracyclines promote survival and fitness in mitochondrial disease models
a, Mitochondrial protein synthesis (%DMSO) versus antibiotic concentration based on band quantification relative to DMSO from Fig. 2b (n = 2 experiments). b, 35S-labelled cysteine and methionine pulse in cybrid cells treated with doxycycline at 1 μM or 10 μM with a 48 hour pre-treatment and 1 hour pulse (n = 2 experiments). c, Western blot of MRPL4 in sgMRPL4 ND1 cybrid cells. d, Western blot of GFM2 in sgGFM2 ND1 cybrid cells.