Extended Data Fig. 3: Biochemical validation of mtSSU assembly modules.
a-c, and e-f, The composition of the B5 (a-c), HB1 (e), and H1 (f) mtSSU assembly clusters. Mitoribosomal complexes were immuno-isolated via FLAG-tagged constituents mS27 (a), mS25 (b), mS40 (c), bS1m (e), and uS10m (f) and separated by sucrose gradient centrifugation. d, g, Sucrose gradient sedimentation analysis of mitoribosomal complexes in mS40 (d) or uS7m (g) deficient cells. H – mtSSU head; B – mtSSU body; HB – mtSSU head-body assembly module.