Extended Data Fig. 10: Cryo-EM 3D reconstruction of a class of particles without mRNA into the channel and the position of eIF4A.

(a to c) Overview of the cryo-EM density map of a class of particles without mRNA into the channel. (d) The inset shows a lack of density for mRNA using mRNA and tRNA superimposed from the 48S structure. (e) Rigid-body fitting of human DHX29 (AlphaFold prediction) into a prior cryo-EM map of a rabbit 43S (EMD:3057)⁴⁴. (f and g) The inset shows that DHX29 and the entry-site eIF4A would overlap and could not coexist.