Extended Data Fig. 9: Protein expression and localization of the MEILB2 dimerization mutants.
From: Structure of a meiosis-specific complex central to BRCA2 localization at recombination sites
(A) and (D) Immunostaining of WT spermatocytes expressing indicated GFP-MEILB2 WT and mutant proteins. (B) and (E) Quantification of GFP foci intensities represented in panel A and panel D, respectively, normalized with the average value of WT. n shows the analyzed foci number pooled from four cells from one electroporated mouse. All analyses used two-tailed t-tests. ****p < 0.0001 (WT vs. Δ1–87: 1.81285 ×10−14). Scale bars: 5 μm. (C) and (F) Immunoblots of testis extracts after electroporating indicated GFP-MEILB2 WT and mutant proteins, blotted with the indicated antibodies (repeated two times). GFP and actin blots in each lane correspond to the same processed sample run on the same gel but blotted sequentially after strip**.