Extended Data Fig. 7: Localization of 3a-EGFP and 3aΔN-EGFP expressed in HEK293 cells.

a, 3a-GFP fluorescence localization in HEK293 cells transfected with 3a-EGFP or 3aΔN-EGFP. Single plane and brightest-point projection are displayed for each. Scale bar, 10 µm. b, Side view and view from the extracellular/lumenal space for dimeric 3a cryo-EM density (gray) from the original (left) and high-resolution (right) maps with unmodeled extended density above the mouth of the pore that may correspond to the N-terminal regions colored in purple. A 3a dimer model is drawn orange (TMD) and pink (CD) inside the density. The position of the final modeled N-terminal residue (S40) is indicated. c, 3a-EGFP or 3aΔN-EGFP field of view with multiple cells imaged using a 20X objective. Scale bar, 20 µm. d, Additional images of cells imaged with the 63X objective with both single plane and brightest-point projections displayed. Scale bar, 10 µm.