Extended Data Fig. 9: Supplements on the phenotypic characterization of D1^cKO/D2 mice.

a, Comparison of spontaneous locomotor behavior between D1^wt/D2 (n = 13) and D1^cKO/D2 (n = 15) mice in term of average and top speed during locomotion episodes (left panel) and motor states architecture (two-sided t-tests, D1^wt/D2 vs. D1^cKO/D2 mice: ns p > 0.05, *p < 0.05). b, Temporal distribution of the locomotor effects of acute amphetamine or saline injections in D1^wt/D2 (NaCl, n = 8; 1 mg/kg, n = 6; 3 mg/kg, n = 7) and D1^cKO/D2 mice (NaCl, n = 6; 1 mg/kg, n = 6; 3 mg/kg, n = 7) and (inserts) total distance traveled over the first 60 min after injection (three-ways ANOVA followed by post-hoc two-ways ANOVA for interaction genotype x time: NaCl, F(23,276) = 1.9; 1 mg/kg, F(23,230) = 5.2; 3 mg/kg, F(23,276) = 0.57; ns p > 0.05, ^##p < 0.01, ^###p < 0.001; and post-hoc two-sided Mann Whitney tests D1^wt/D2 vs. D1^cKO/D2 mice on distance traveled over 60 min: ns p > 0.05, *p < 0.05, ***p < 0.001). c, Temporal evolution of traveled distance across repeated 10 mg/kg cocaine injection in D1^wt/D2 (black) and D1^cKO/D2 mice (orange) (three-ways ANOVA followed by post-hoc two-ways ANOVA for interaction genotype x time: Day1, F(26,806) = 1.9; Day2, F(26,806) = 2.29; Day3, F(26,806) = 6.55; Day4, F(26,806) = 4.96; Day5, F(26,806) = 6.08; Day6, F(26,806) = 4.27; Day14, F(26,806) = 2.72; ^###p < 0.001). Data are presented as mean values ± SEM. Detailed statistics are displayed in Supplementary Table 1. Source data are provided as a Source Data file.

Source data