Extended Data Fig. 2: MAGUK and Gephyrin puncta numbers in OPCs increase during early development and exhibit small differences across CNS regions.
(a) Quantification of MAGUK (green) and Gephyrin (magenta) puncta number per OPC in the spinal cord at different ages by IHC. From left, n = 20, 18, 8, 17, 24, and 8 cells from at least 6 fish per condition. (b) Quantification of MAGUK (green) and Gephyrin (magenta) puncta number per OPC at 3 dpf across different CNS regions. FB&MB, forebrain and midbrain; HB, hindbrain; SC, spinal cord. From left, n = 34, 59, 43, 34, 18, and 40 cells from at least 10 fish per condition. (c) Schematic model showing the neuron-rich (NR) and axon & synapse-rich (AR) regions and two representative images showing OPCs with soma localized to NR (top) and AR (bottom) regions in Tg(olig1:Kalta4,10xUAS:myrGFP) spinal cord. (d) Quantification of MAGUK (green) and Gephyrin (magenta) puncta number per OPCs in NR or AR regions at 2-3 dpf. From left, n = 14, 5, 15, and 10 cells from at least 5 fish per condition. All data are represented as mean ± SEM; N.S., not significant (exact p values are provided in Source Data); (a-b) Kruskal-Wallis test followed by Dunn’s multiple comparison test; (d) two-sided Mann-Whitney test; scale bar 5 μm.