Supplementary Figure 2: Expression and functional effects of Arch3.0 in the BLA.

a, b, Sample images showing eYFP expression in the BLA (a) and the prelimbic mPFC (b) after mice were injected with AAV-CaMKIIα-Arch3.0-eYFP in the BLA (n = 2 mice). The scale bars in (a) and (b) are 200 μm and 50 μm, respectively. c, Sample current-clamp trace showing that yellow light illumination inhibits electrical-evoked action potential firing in an Arch3.0-eYFP expressing BLA neuron (n = 4 cells). d, Sample voltage-clamp traces showing yellow light inhibition of electrical-evoked EPSCs in pyramidal cells of the prelimbic mPFC in mice that are injected with AAV-CaMKIIα-Arch3.0-eYFP or AAV-CaMKIIα-NpHR3.0-eYFP in the BLA. e, Mean eEPSC amplitudes for the experiments in (d) (p = 0.0187, t = 3.429 and p = 0.0004, t = 8.464 for Arch and NpHR, respectively). Note that 2-3 times the threshold stimulus intensity was used to induce eEPSCs and bicuculline (10 μM) was added to the bath solution to isolate direct monosynaptic glutamatergic inputs from the BLA to mPFC pyramidal neurons without incorporating effects of yellow light on GABAergic feedforward inhibition of pyramidal cell activity. Data are presented as mean ± SEM. A two-tailed paired t-test was used for e, where numbers in parentheses denote numbers of cells.