Supplementary Figure 7: Flow cytometric analysis of adult mouse forebrain and comparison to single-cell RNA-seq data from different brain regions.

a–c Dot blots illustrating nuclei from adult forebrain stained for flow cytometry with Alexa488 conjugated secondary antibodies. a. Displayed are representative blots for experiments without antigen specific primary antibody and b. with antibodies recognizing the post-mitotic neuron marker NeuN²² (n = 3, average ± SEM). c. NeuN negative nuclei were sorted for ATAC-seq experiments and purity (>98%) was confirmed by flow cytometry of the sorted population. d. Relative composition of different forebrain regions derived from single cell RNA-seq shows region specific differences¹⁹. e Relative composition derived from snATAC-seq (compare to Fig.2c) of adult forebrain shows values in between.