Extended Data Fig. 7: Nose touch response of synaptically localized TeNL.
From: Neural engineering with photons as synaptic transmitters
(a) Fluorescence micrograph i) of the synaptically localized Nanolantern, fused to SNG-1 Synaptogyrin and the other transgenes. Cyan arrowheads point towards the presumptive synapses indicated by high SNG-1::TeNL intensities. Scale bars = 40 μm. ii) Calcium saturated bioluminescence micrograph of the SNG-1::TeNL. Image is representative of a set of 4 derived from 2 independent lines. (b) Nose touch avoidance response of an animal carrying the ASH-specific eat-4(loxP) mutant allele in absence and presence of indicated cofactors, coexpressing synaptic SNG-1::TeNL in ASH and ChR2-HRDC in AIB and AVA, displayed as a grid plot, color-coded according to its outcome (blue=positive response, gray=negative response to nose touch). (c) Summary of the scores for the nose touch experiment on 30 animals in all conditions tested. Each blue dot corresponds to the average from ten touch tests per animal. Only for display purposes, a scatter of 10% was applied to each datapoint to avoid overlap. Horizontal bar indicates median, vertical bar indicates 95% confidence interval on the median. Floating axis indicates the paired median difference, derived from bootstrap** 100 independent distributions from the experimental data set. Red point indicates median, red vertical bar indicates 95%CI. Overlap of the CI with zero indicates low effect size and likely statistically insignificant distributions. Numbers on gray brackets indicate the two-sided p-value derived from a Wald test.