Extended Data Fig. 10: YFV∆CME-mVenus tracing from pontine nuclei to cerebellum.
From: Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D
(a) Image of a brain section after injection of AAV-tTA, AAV-TRE-C-prM-E-NS1 and YFV∆CME-mVenus into pontine nucleus and adjacent reticular tegmental nucleus. (b) mVenus-positive cells in the granular layer of the cerebellar cortex. The sections were counterstained with a marker for Purkinje cells, PCP4 (red), and DAPI (Blue). The experiments were repeated 4 times with similar results. The images are tile scans of brain sections. The blue color is counterstaining with DAPI.