Extended Data Fig. 3: Characterization of human intestinal fibroblasts and the jejunal grafts reconstructed on human scaffold.

a, Representative immunofluorescent images of primary human jejunal fibroblasts [patient 2] showing fibronectin (F-NEC, yellow), vimentin (VIM, green), fibroblast surface protein marker-1 (FSP-1, magenta), laminin alpha 5 (LAMA5, cyan) and alpha-smooth muscle actin (αSMA, red). b, Representative images showing a blank scaffold (left), after stromal cell injection (middle) and during organoid seeding (right) on a mounting stage. c, Photos showing the media reservoir (left), peristaltic pump (middle) and the perfusion plate (right) of the bioreactor. d, Representative immunofluorescent staining of an engineered graft [patient 7 fibroblasts; patient 2 colon scaffold] using the indicated antibodies. e, Electron micrograph showing a monolayer of epithelial cells on a graft [SI scaffold - patient 5]. All scale bars represent 50μm. All organoids used in this figure were from patient 2. Images are representative of cells from 3 independent experimental cultures (a) or 4 grafts (d,e).