Extended Data Fig. 5: Importance of RORγt+ cells in the anti-tumor immune response.
From: Recruitment and activation of type 3 innate lymphoid cells promote antitumor immune responses
Flow cytometry analysis of a, RORγt+IL-17+ Th17 and b, CCR6+ and NKP46+ IL-17+ ILC3s cells from WT mice intestine treated with PBS, GSK805 or Digoxin n = 5 and of c, RORγt+ Th17 and d, CCR6+ ILC3 from TC-1 tumor seven days after CDDP treated with Digoxin or GSK805, n = 7. Numbers for 300 000 CD45+ cells. e-p Flow cytometry analysis of immune cells infiltrating TC-1 tumors one, three and seven days after a CDDP and or Digoxin treatment. Numbers are shown for 300 000 total viable cells from each TC-1 tumor. n = 7. Numbers of e, ILC3s, f, ILC2s, g, ILC1s, h, NKs, i, CD8 + cells, j, CD4 + cells, k, B cells, l, DCs, m, macrophages, n, M-MDSCs, o, PMN-MDSCs and p, eosinophils. Results are shown as mean ± SD and are representative of three independent experiments. Statistical significance was determined using one-way anova (a–d) and two-way anova and Tukey’s multiple comparison test (e–o).