Extended Data Fig. 6: PLZF expression is reduced in the absence of BCL-6.
From: The transcription factor BCL-6 controls early development of innate-like T cells
a, qPCR analysis showing the transcript levels of Zbtb16 in sorted ST0 and ST1 cells from control and Bcl6Δ/Δ mice. Graphs represent the mean ± SEM. n = 3 independent experiments with 3–5 pooled thymi for each genotype. b, FACS plots showing the expression of PLZF protein in ST0 (upper panel) and ST1 (lower panel) cells from the indicated mouse strains. Graphs show the mean percentage ± SEM of PLZF+ cells in the indicated populations. n = 5 (ST0) and n = 8 (ST1) independent experiments. c, Graph showing the geometric MFI of PLZF expression in PLZF-expressing ST0 cells. Graphs represent the mean ± SEM. n = 4 independent experiments. d, Histograms showing the expression of PLZF in ST2 and ST3 cells in the indicated mouse strains. Graphs show the mean percentage ± SEM of PLZF— cells. n = 5 independent experiments. e, Histograms showing the expression of CD69 and CD5 in ST0 iNKT cells from the indicated mouse strains. The corresponding graphs show the mean geometric MFI ± SEM. n = 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical analysis was performed with one-sample t-test (a), two-tailed unpaired t-test (b, d, and e) or two-tailed paired t-test (c).