Extended Data Fig. 2: Cell line engineering scheme in MB231 and HEK293 cells, and flow cytometry quantitation of inducible expression in selected HEK293 clones.
From: Nonmonotone invasion landscape by noise-aware control of metastasis activator levels
![Extended Data Fig. 2](http://media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41589-023-01344-z/MediaObjects/41589_2023_1344_Fig8_ESM.jpg)
(a) Cell line engineering steps and corresponding annotations for different sets of engineered cells, indicating the chosen parental LP clones and corresponding mNF clones. Low- and high-noise clones of both mNF-GFP and mNF-BACH1 clones were labelled GL, GH and BL, BH, respectively. (b) Representative dose-responses of fluorescence intensity histograms from low-noise mNF-GFP (GL), mNF-BACH1 (BL) and high-noise mNF-GFP (GH), mNF-BACH1 (BH) 293 clones measured at 0, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 100 ng ml−1 Dox levels, respectively. (c) Dose-responses of mean fluorescence intensity (MFI) for low-noise mNF-GFP (GL), mNF-BACH1 (BL) and high-noise mNF-GFP (GH), mNF-BACH1 (BH) 293 clones (n = 3). (d) Dose-responses of coefficient of variation (CV) from low-noise mNF-GFP (GL), mNF-BACH1 (BL) and high-noise mNF-GFP (GH), mNF-BACH1 (BH) 293 clones (n = 3).