Extended Data Fig. 3: Expression of H3K36 KMTases and the role of H3K36me2 during male germline development.

a, Dynamics of expression of H3K36 KMTases across prenatal male germ cell development. FPKM values were extracted from RNA-seq data from a previous study³⁴. The window of global de novo DNAme is shaded in yellow. b, Breeding scheme to obtain germline-specific Nsd1 KO PSG and sorting strategy for PSG purification, as in Extended Data Fig. 1c. A microscopy image of GFP expression in E16.5 testis is shown (scale bar, 500 μm) as well as a representative FACS plot for control testis, with PSG representing 3.1% of total testis cells in this experiment. c, Tracks showing coverages and junctions of RNA-seq reads over the Nsd1 locus in Nsd1 HET and KO E16.5 PSG. Note that the exon between the two loxP sites (shaded in yellow) is efficiently excised (>99%) in the KO. d, Bar graph showing the % genomic coverage of H3K36me2 and H3K36me3 in Nsd1 HET versus KO E16.5 PSG. e, Scatterplot showing the correlation of H3K9me3 (RPKM) over 10-kb genomic bins in Nsd1 HET versus KO E16.5 PSG. f, Scatterplot showing H3K9me3 versus H3K36me2 enrichment (RPKM over 10-kb genomic bins) in Nsd1 KO E16.5 PSG.