Extended Data Fig. 6: Location stereotypy and concordance are robust to systematic variation in cell and nuclear shape.
From: Integrated intracellular organization and its variations in human iPS cells
a. Heat map of the 2D pixel-wise Pearson correlation matrix for all pairs of cellular structure PILRs among all cells in the 8-dimensional sphere. Each entry in this matrix represents the correlation between the PILR of two cells. Coloured triangles to the left of, and the thicker black lines within, the matrix indicate the regions (blocks) of the matrix corresponding to cells with the indicated tagged structure. The dimensions of each block correspond to the number of cells. b. Average correlation matrix. Left: the location stereotypy for a cellular structure is the average of all the values in the blocks along the diagonal of the correlation matrix in (a). The numbers on the right indicate structures ranked by their stereotypy from greatest to least. Right: the location concordance for any two pairs of structures is the average of all the values in the corresponding structure pair block in the correlation matrix in (a). The diagonal of the concordance heat map corresponds to the stereotypy. Arrows indicate examples of the relationships between the heat maps in (a) and (b). c. Stereotypy heat maps for each of the eight shape modes (SM). Each row represents a different cellular structure and each column represent the nine binned map points along each shape mode (Fig. 2b). d. Concordance heat maps for the −2σ and 2σ shape space map points for each of the eight shape modes. The lower and upper triangles represent the −2σ and 2σ map points, respectively. Numbers of cells and heat map data in Supplementary Data 1. Colour bars on the left of heat maps indicate the cellular structure.