Extended Data Fig. 4: Exposure to pathogenic Serratia bacteria induces pheromone response of AWA.
From: Pathogenic bacteria modulate pheromone response to promote mating
a, Schematic of exposure to different bacteria strains for calcium imaging analysis. b,c, Traces of GCaMP6 signals in AWA neurons of wild-type adult hermaphrodites in response to ascaroside pheromones after 4–6 h exposure to different bacteria strains (b) and quantification of average GCaMP6 signals in AWA (c) during pheromone stimulation in b. Phe, pheromone mixture of ascr#2, ascr#3 and ascr#5 (1 μM each). Lines in traces, mean; shades, s.e.m. (b). Fb is baseline, defined as average GCaMP6 signal in the first 30 s. In box plots, the centre line shows the median, box edges delineate 1st and 3rd quartiles and whiskers extend to minimum and maximum values.Numbers in parentheses, number of different worms (c). Dots, average GCaMP6 signals of individual worms during pheromone stimulation (c). P values are derived from Kruskal-Wallis test with Dunn’s multiple comparisons test, asterisks indicate significant difference, *** P < 0.001; ns, not significant. P values are shown in Source data. d, e, Schematics for TRAP-RNAseq (d) and for exposure to different bacteria strains for TRAP-RNAseq analysis (e).