Extended Data Fig. 3: B. xylanisolvens transplantation alleviates nicotine-accelerated NASH.
From: Gut bacteria alleviate smoking-related NASH by degrading gut nicotine
HFHCD-fed SPF mice were treated with PBS, B. xylanisolvens colonization, nicotine water, nicotine water plus B. xylanisolvens colonization, and nicotine water plus nicX knock-out B. xylanisolvens colonization for 20 weeks (n = 6 mice/group). a, Faecal B. xylanisolvens abundance analyses of mice by qPCR. b, Ileal nicotine concentrations. c, Body weight gain. d, Body mass composition. e, Liver weights. f, Liver weight–to–body weight ratios. g, h, Serum ALT (g) and AST (h) levels. i, Hepatic TG content. j, Serum TG content. k, Hepatic CE content. l, Serum CE content. m, Serum NEFA content. n–r, Histology scores of hepatic steatosis (n), lobular inflammation (o), ballooning (p), NAFLD activity (q), and fibrosis stage (r). s–u, Relative mRNA levels of genes related to hepatic lipid metabolism (s), inflammation (t) and fibrosis (u). Data are the means ± s.e.m. c, e–i, k–m, One-way ANOVA with Tukey’s post hoc test. a, b, j, One-way ANOVA with Dunnett’s T3 post hoc test. d, n–u, Kruskal-Wallis test with Dunn’s test.