Extended Data Fig. 5: Flow cytometric analyses of the spleen and BALF after CVB4 infection.

Flow cytometry and FACS analyses following CVB4 infection of Lx9c11^−/− and C57BL/6j (WT) mice. (A) Representative dot plots from flow cytometric analyses showing gating strategy for NK cells in the spleens of WT and Lx9c11^−/− mice on day 4 of CVB4 infection, also noted in the Methods section. Percentages (B) and numbers (C) of NK cells in the spleen of WT and Lx9c11^−/− mice on day 1 following CVB4 infection. Percentages (D) and numbers (E) of NK cells in the spleen of WT and Lx9c11^−/− mice on day 4 of CVB4 infection. For panels B–E; data are shown as n = 4–5 individual mice/group and mean ± SD from 2 independent experiments. Statistical significance was determined by 2-tailed unpaired t-test. (F) CD11b⁺ F480⁺ macrophages, (G) CD11b⁺ Ly6G⁺ neutrophils (H) CD8⁺ CD44^hi CD3⁺ T cells (I), CD4⁺ CD44^hi CD3⁺ T cells in the spleen on day 4 of infection. Quantitation of cells in WT and Lx9c11^−/− BALF on day 7 of CVB4 infection, gating strategy shown in Fig. 2q and in the Methods section. Numbers of (J) CD64⁺ monocytes/macrophages, (K) Ly6C⁺CD64⁺ monocyte subset, (L) Ly6C⁻CD64⁺ macrophage subset, (M) CD69⁺ CD8⁺ T cells, (N) activated/memory phenotype CD44hi⁺ CD8⁺ T cells, (O) Activated/memory phenotype CD44^hi CD4⁺ T cells. For panels F-O; data are extended from Fig. 2 and shown as individual mice and mean ± SD n = 4–5 individual mice/group, from 2 independent experiments, and statistical significance was determined by one-way ANOVA.