Extended Data Fig. 6: Growth kinetics of artificially generated GFP-expressing viruses.
From: Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation
The GFP-expressing D614G and D614G/P681R mutant viruses were generated by reverse genetics. These viruses (100 TCID50 for Vero and VeroE6/TMPRSS2 cells, 100 or 1,000 TCID50 for Calu-3 cells) were inoculated into cells. The viral RNA copy number of in the culture supernatant (a) and the level of GFP-positive cells (the percentage of GFP-positive cells for Vero and VeroE6/TMPRSS2 cells; the GFP intensity per well for Calu-3 cells) (b) are shown. Data are mean ± S.D. (c) Representative images of Calu-3 cells infected with GFP-expressing viruses (100 TCID50). Areas enclosed with circles are enlarged in the right panels. Assays were performed in quadruplicate. Statistically significant differences (*P < 0.05) versus the D614G virus were determined by two-sided Student’s t test. NS, no statistical significance.