Extended Data Fig. 9: MBaMV accessory genes do not antagonize human IFN induction.
From: Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus
a) HEK 293 T cells were co-transfected with an ISG54-ISRE-FLuc plasmid and either MBaMV P, MBaMV V, MeV V, MeV P, or ZIKV NS5. Cells were treated with human IFNb and ISRE induction was measured by luciferase assay. HEK 293 T cells were co-transfected with an IFNb-FLuc plasmid, an IFN promoter stimulant b) RIG-I, c) MDA5, and d) MAVS, and MeV P, MeV V, MBaMV P, MBaMV V, or HCV NS3/4 A. IFN induction was measured by luciferase assay in 2 independent experiments with 3 technical replicates (mean + /- S.D.). The specific p values (* p < 0.5; **** p =< 0.0001) were calculated using a one-way ANOVA with a Dunnett’s multiple comparisons test.