Extended Data Fig. 7: Global DPC induction and repair is not affected by CSB loss.
From: Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB
(a) Representative images from RRS assays in WT, CSB−/−, ERCC1−/− and XPG−/− RPE1 cells treated with formaldehyde, representative of 3 independent experiments. (b) RRS assays in WT SPRTN-ΔC, CSB−/− and SPRTN-ΔC/CSB−/− RPE1 cells treated with formaldehyde and released for the indicated times. Mean intensities of replicates are displayed as black dots with a mean of those averages shown as line; n = 3 replicates error bars ± SEM. (c) Volcano plot from PxP-MS in WT RPE1 cells treated with formaldehyde compared with cells treated with formaldehyde and released for 6 h. (d) Heat map displaying Z-scored intensities for DNA-crosslinked proteins identified by PxP-MS after formaldehyde treatment and release for the indicated times in WT and CSB−/− RPE1 cells. Intensities are displayed for three replicates per condition. Source numerical data are available in source data.