Cell death is an important biological process whose experimental detection and measurement can be difficult, especially when examining many conditions in parallel. The interpretation of cell death data is complicated by the diversity of measurement techniques and lack of standardized methods in the field. Here, we offer tips to help interpret cell death experiments.
References
Chan, G. K., Kleinheinz, T. L., Peterson, D. & Moffat, J. G. PLoS One 8, e63583 (2013).
Forcina, G. C., Conlon, M., Wells, A., Cao, J. Y. & Dixon, S. J. Cell Syst. 4, 600–610 e606 (2017).
Gelles, J. D. et al. Dev. Cell 51, 277–291 e274 (2019).
Richards, R. et al. Nat. Chem. Biol. 16, 791–800 (2020).
Harris, L. A. et al. Nat. Methods 13, 497–500 (2016).
Schwartz, H. R. et al. Cell Rep. 31, 107800 (2020).
Hafner, M. et al. Cell Chem. Biol. 26, 1067–1080 e1068 (2019).
Hafner, M., Niepel, M., Chung, M. & Sorger, P. K. Nat. Methods 13, 521–527 (2016).
Ben-David, U. et al. Nature 560, 325–330 (2018).
Elliott, J. T. et al. ALTEX 34, 201–218 (2017).
Haverty, P. M. et al. Nature 533, 333–337 (2016).
Rosslein, M. et al. Chem. Res. Toxicol. 28, 21–30 (2015).
Vaux, D. L. Nature 492, 180–181 (2012).
Gong, Y. N. et al. Cell 169, 286–300 e216 (2017).
Shah, R., Shchepinov, M. S. & Pratt, D. A. ACS Cent. Sci. 4, 387–396 (2018).
Acknowledgements
We thank members of the Dixon laboratory and L. Magtanong for comments on the manuscript. This work was also supported by an award from the National Institutes of Health (NIH) (2R01GM12292306) to S.J.D.
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S.J.D. is a co-founder of Prothegen Inc., a member of the scientific advisory board for Hillstream Biopharma and holds patents related to ferroptosis. M.J.L. declares no competing interests.
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Dixon, S.J., Lee, M.J. Quick tips for interpreting cell death experiments. Nat Cell Biol 25, 1720–1723 (2023). https://doi.org/10.1038/s41556-023-01288-5
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DOI: https://doi.org/10.1038/s41556-023-01288-5
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