Extended Data Fig. 3: Nuclear lamina compacts together with chromosomes in the absence of microtubules and F-actin.
a, Live porcine oocytes expressing EGFP-Lamin B1 and H2B-mCherry (chromosomes) treated with DMSO or nocodazole and cytochalasin D. Z-projections, 6 sections every 3 µm. b, Immunofluorescence airyscan images of human oocytes before and at different time points after NEBD. Magenta, DNA (Hoechst); green, Lamin A/C; F-actin (not shown); alpha-tubulin (not shown). Representative examples of 53 immunolabeled human oocytes. c, Quantification of the largest pairwise distance calculated from the chromosome signal in control or nocodazole and cytochalasin D co-treated porcine oocytes before NEBD and right before drug (nocodazole and cytochalasin D) washout. Box plot shows median (horizontal black line), mean (small black squares), 25th and 75th percentiles (boxes), 5th and 95th percentiles (whiskers) and 1st and 99th percentiles (dots). Scale bar, 10 µm. Time, minutes (min) after NEBD. Number of oocytes in brackets. Data from three independent experiments unless otherwise specified. Source numerical data are available in source data.