Extended Data Fig. 2: Additional patterns of chromosome clustering in porcine oocytes.
a, Bar plot showing percentage of porcine oocytes with different levels of chromatin clustering around the nucleolus prior to NEBD. b, Porcine oocytes expressing H2B-mCherry (chromosomes) and EGFP-MAP4 (not shown). Examples of the classes of chromatin configurations presented in a. Yellow arrowheads point at the nucleolus. Dashed circles demark nucleolus. Z-projection panels, 15 sections every 3 µm. c, Bar plot showing percentage of porcine oocytes that fully or partially clustered their chromosomes after NEBD. d, Porcine oocytes expressing EGFP-MAP4 (microtubules) and H2B-mCherry (chromosomes). Examples of partial chromatin clustering after NEBD. Z-projections, 15 sections every 3 µm. Scale bar, 10 µm. Time, minutes (min) from NEBD. Number of oocytes indicated with n. Data from three independent experiments. Source numerical data are available in source data.