Supplementary Figure 3: Recombination efficiency of N1Cre/Confetti mice at different embryonic developmental times and tamoxifen doses.

Representative single Z-stacks of wholemount immunostaining of embryonic mammary buds from N1Cre/Confetti mice induced with tamoxifen at E13.5 (a), and E17.5 (b) at doses 0.1mg (upper images), or 2 µg (lower image), and analysed 48h later. Immunostaining for K5 is shown in white. Scale bars correspond to 100 μm, and to 50 μm in the magnifications; n=20 glands in 11 embryos (a) and 31 glands in 13 embryos (b). c-e. Quantification of the number of cells per coloured clone (c), total number of cells per gland (d) or number of colours per gland (e) at 48h-chase from females induced at the indicated time points during embryogenesis with full dose (0.1 mg) or diluted doses (0.05 mg or 2 µg) of tamoxifen; n=98 glands in 30 embryos. p=0.0004 and p=0.036 (c); p<0.0001 and p=0.004 (d); p=0.0001 and p=0.0118 (e). Graphs indicate average values ± SEM. *p<0.05, **p<0.01, ***p< 0.001, ****p< 0.0001 using two-tailed non-parametric Mann Whitney t-test. Source data are available in Supplementary Table 1.