Supplementary Figure 5: Nucleosome-free regions at cohesin loading sites are jointly maintained by the Scc2–Scc4 and RSC complexes.

A region around the RPL19B promoter was chosen to display nucleosome and Scc2-ChIP profiles in the indicated strains at 25 °C and 37 °C. The analysis confirms that maintenance of the RPL19B promoter nucleosome-free region, like that at the RPS8B promoter shown in Figure 4a, requires both Scc2–Scc4 and RSC complex function.