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Cas9-triggered chain ablation of cas9 as a gene drive brake

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Figure 1: Schematics and proof of principle of CATCHA.

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Acknowledgements

We thank D. Luginbuhl for generating transgenic flies, F. Port for providing gRNA flies, and J. Luo, T. Mosca, X. Wang, J. Li and J. Lui for advice and critical comments. This study was supported by an NIH grant (R01-DC005982) to L.L. X.J.G. was supported by an Enlight Foundation Interdisciplinary Fellowship. L.L. is an HHMI investigator.

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Author notes

  1. **ao**g J Gao

    Present address: Present address: Division of Biology, California Institute of Technology, Pasadena, California, USA.,

  2. Bing Wu and **ao**g J Gao: These authors contributed equally to this work.

Authors and Affiliations

  1. Howard Hughes Medical Institute and Department of Biology, Stanford University, Stanford, California, USA

    Bing Wu, Liqun Luo & **ao**g J Gao

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  1. Bing Wu
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  2. Liqun Luo
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Correspondence to Liqun Luo.

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Supplementary Figures 1 and 2, Supplementary Table 1 and Supplementary Methods (PDF 1170 kb)

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Wu, B., Luo, L. & Gao, X. Cas9-triggered chain ablation of cas9 as a gene drive brake. Nat Biotechnol 34, 137–138 (2016). https://doi.org/10.1038/nbt.3444

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