Extended Data Figure 10: Disruption of TORC1–TORC1 contact interfaces abolishes TOROID assembly.
From: TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity
a, Quantitative analysis of TORC1 focus disassembly after dilution of stationary-phase WT cells expressing GFP–Kog1 into either fresh CSM containing rapamycin, CMB4563, or drug vehicle (Mock). b, Quantitative analysis of TORC1 focus formation after treatment of late log-phase WT cells expressing of GFP–Kog1 with either rapamycin, CMB4563, or drug vehicle (Mock). b, c, Black diamonds, mock treatment; grey squares, rapamycin treatment (200 nM); grey triangles, CMB4563 treatment (800 nM). Data are mean ± s.d. and represent three independent experiments (150–800 cells each). c, Representative confocal images of WT or Δgtr1 Δgtr2 cells expressing GFP–Tor1 or Tor1D330::3xGFP in exponential or stationary growth phases.