Abstract
Tear fluid collected from healthy children and adults, was tested for its capacity to inhibit Chlamydia trachomatis, serotype I, to form inclusions in McCoy cell cultures. Pooled tear fluid added to such cultures reduced the chlamydial inclusion count even at concentrations of 1%. The inhibitory activity was concentration-dependent. The chlamydial inhibitory factor has a molecular weight of <10 000 dalton and the principle is heat-stable. The antichlamydial factor seems to affect the attachment of the elementary body (EB) to the host cell surface, while no effect on the intracellular development and reproduction of the chlamydiae could be demonstrated. The activity could not be explained by the presence of antichlamydial antibodies.
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References
Allansmith MR : Defence of the ocular surface. Int Ophthalmol Clin 1979, 19: 93–109.
Fleming A : On a remarkable bacteriolytic element found in tissues and secretions. Proc Roy Soc 1922, 43: 306–17.
Thompson, R and Gallardo E : The antibacterial action of tears on staphylococci. Am J Ophthalmol 1941, 34: 635–40.
Friedland BR, Anderson DR, Foster RK : Non-lysozyme antibacterial factor in human tears. Am J Ophthalmol 1972, 74: 52–9.
Ford LC, Delange RJ, Petty RW : Identification of a nonlysozymal bacterial factor (β-lysin) in human tears and aqueous humour. Am J Ophthalmol 1976, 81: 30–3.
Jensen RS, Tew SG, Donaldson DM : Extracellular beta-lysin and muramidase in body fluids and inflammatory exudates. Proc Soc Broc Med 1967, 124: 545–9.
Broekhuyse RM : Tear lactoferrin—a bacteriostatic and complexing protein. Invest Opthalmol 1974, 13: 550–4.
Selsted ME and Rafael J : Isolation and purification of bactericides from human tears. Exp Eye Res 1982, 34: 305–18.
Wølner-Hansen P, Weström L, Mårdh P-A : Influence of amniotic fluid on the formation of chlamydial inclusions in McCoy cell cultures. In Mårdh P-A et al. eds. Amsterdam: Elsevier Biomed Press. 1982, 283–286.
Elbagir AN, Ching C, Osman N, Axemo P, Bergström S, Mårdh P-A : Notes on antichlamydial activity of amniotic fluid. In Mårdh, P-A et al., eds. Proceedings of the European Society for Chlamydial Research. Stockholm: Almqvist & Wiksell International. 1988,128.
Mårdh P-A, Colleen S, Sylwan J : Inhibitory effect on the formation of chlamydial inclusion in McCoy cells by seminal fluid and some of its components. Invest Urol 1980, 17: 510–13.
Ripa KT and Mårdh P-A : Cultivation of Chlamydia trachomatis in cycloheximide-treated McCoy cells. J Clin Microbiol 1977, 6: 328–31.
Treharne JD, Darougar S, Jones BR : Modification of microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infections. J Clin Patho1 1977, 30: 510–17.
Robert NS, James JF, Farris RL, Irwin DM : The effect of collection technique on tear composition. Invest Olphthal Vis SCi 1984, 25: 374–7.
Soltesz V, Zeeberg B, Mårdh P-A : Toxic effect of sampling materials on the reproduction of Chlamydia trachomatis in McCoy cell cultures. Proceedings of the SSGM 5th meeting. 1988, Abs 16.
Hackstadt T : Identification and properties of chiamydial peptides that bind eucaryoticcell surface components. J Bacteriol 1986, 13–20.
Kueppers F : Proteinase inhibitors in human tears. Biochem Biophys Acta 1971, 229: 845–9.
Carrol SF and Martinez RJ : Role of rabbit lysozyme in vitro serum and plasma serum bactericidal reactions against Bacillius subtilis. Infect Immun 1979, 25: 810–19.
Sugarman B and Agbor P : The binding of Chlamydia trachomatis and zinc to McCoy cells (mouse fibroblasts). Infection 1987, 15: 39–43.
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Elbagir, A., Stenberg, K., Fröman, G. et al. Antichlamydial activity of tear fluid. Eye 3, 854–859 (1989). https://doi.org/10.1038/eye.1989.131
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DOI: https://doi.org/10.1038/eye.1989.131
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