Abstract
Chromosome XV was one of the last two chromosomes of Saccharomyces cerevisiae to be discovered1. It is the third-largest yeast chromosome after chromosomes XII and IV, and is very similar in size to chromosome VII. It alone represents 9% of the yeast genome (8% if ribosomal DNA is included). When systematic sequencing of chromosome XV was started, 93 genes or markers were identified, and most of them were mapped2. However, very little else was known about chromosome XV which, in contrast to shorter chromosomes, had not been the object of comprehensive genetic or molecular analysis. It was therefore decided to start sequencing chromosome XV only in the third phase of the European Yeast Genome Sequencing Programme, after experience was gained on chromosomes III, XI and II (refs 3–5). The sequence of chromosome XV has been determined from a set of partly overlap** cosmid clones derived from a unique yeast strain, and physically mapped at 3.3-kilobase resolution before sequencing. As well as numerous new open reading frames (ORFs) and genes encoding tRNA or small RNA molecules, the sequence of 1,091,283 base pairs confirms the high proportion of orphan genes and reveals a number of ancestral and successive duplications with other yeast chromosomes.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dujon, B., Albermann, K., Aldea, M. et al. The nucleotide sequence of Saccharomyces cerevisiae chromosome XV. Nature 387 (Suppl 6632), 98–102 (1997). https://doi.org/10.1038/387s098
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/387s098
- Springer Nature Limited
This article is cited by
-
Profilin is required for Ca2+ homeostasis and Ca2+-modulated bud formation in yeast
Molecular Genetics and Genomics (2013)
-
Systematic discovery of unannotated genes in 11 yeast species using a database of orthologous genomic segments
BMC Genomics (2011)
-
Distribution of genes and recombination in wheat and other eukaryotes
Plant Cell, Tissue and Organ Culture (2005)
-
Gene assembly consisting of small units with similar amino acid composition in the Saccharomyces cerevisiae genome
Mycoscience (2003)