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Expression of rabies virus glycoprotein from a recombinant vaccinia virus

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Abstract

Rabies is one of the oldest diseases known to man, but its successful control has remained elusive. Although effective vaccines of tissue culture origin against rabies do exist1, such preparations are expensive. Live vaccinia virus (VV) recombinants expressing influenza or hepatitis B antigens have recently been used to immunize against these diseases2–4. We have now used this approach to produce a novel rabies vaccine. We first altered the rabies glycoprotein cDNA5 by site-directed mutagenesis and removed the poly(dG) tail. We then aligned the modified cDNA with an early VV promoter sequence inserted within a cloned copy of the vaccinia thymidine kinase gene and transfected this plasmid into VV-infected cells. Recombination between the virus and the plasmid resulted in a recombinant virus harbouring the rabies glycoprotein cDNA. Inoculation of rabbits with the live recombinant virus induced high titres of rabies virus-neutralizing antibodies, and scarification with the recombinant VV protected mice against challenge with street rabies virus.

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Author notes

  1. R. Lathe

    Present address: AFRC Animal Breeding Research Organisation, King's Buildings, West Mains Road, Edinburgh, EH9 3JQ, UK

Authors and Affiliations

  1. Transgène S. A., 11 rue de Molsheim, 67000, Strasbourg, France

    M. P. Kieny, R. Lathe, S. Skory, D. Schmitt & J. P. Lecocq

  2. Laboratoire de Virologie et U.74 INSERM, 3 rue Koeberlé, 67000, Strasbourg, France

    R. Drillien & D. Spehner

  3. Wistar Institute, 36 Street at Spruce, Philadelphia, Pennsylvania, 19104, USA

    T. Wiktor & H. Koprowski

Authors
  1. M. P. Kieny
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  2. R. Lathe
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  3. R. Drillien
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  4. D. Spehner
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  5. S. Skory
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  6. D. Schmitt
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  7. T. Wiktor
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  8. H. Koprowski
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  9. J. P. Lecocq
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Kieny, M., Lathe, R., Drillien, R. et al. Expression of rabies virus glycoprotein from a recombinant vaccinia virus. Nature 312, 163–166 (1984). https://doi.org/10.1038/312163a0

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  • DOI: https://doi.org/10.1038/312163a0

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