Abstract
The structure of the operator-binding domain of the λ repressor has been determined at 3.2 Å resolution. This domain contains an extended N-terminal arm and five α-helices. Model-building studies of the repressor–operator complex suggest that α-helices, especially the N-terminal parts of these helices, may provide a useful surface for protein–DNA interactions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ptashne, M. et al. Cell 19, 1–11 (1980).
Johnson, A. D. et al. Nature 294, 217–223 (1981).
Sauer, R. T. & Anderegg, R. Biochemistry 17, 1092–1100 (1978).
Pabo, C. O., Sauer, R. T., Sturtevant, J. M. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 1608–1612 (1979).
Sauer, R. T., Pabo, C. O., Meyer, B. J., Ptashne, M. & Backman, K. C. Nature 279, 396–400 (1979).
Johnson, A. D. thesis, Harvard Univ. (1980).
Pabo, C. O. thesis, Harvard Univ. (1980).
Bricogne, G. Acta crystallogr. A32, 832–847 (1976).
Diamond, R. Computing in Crystallography (eds Diamond, R., Ramaseshan, S. & Venkensatan, K.) (Indian Academy of Science (1981).
Maniatas, T. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 70, 1531–1535 (1973).
Humayun, Z., Kleid, D. & Ptashne, M. Nucleic Acids Res. 4, 1595–1607 (1977).
Johnson, A. D. J. mol. Biol. (in the press).
Pabo, C. O., Krovatin, W., Jeffrey, A. & Sauer, R. T. Nature 298, 441–443 (1982).
Sauer, R. T. thesis, Harvard Univ. (1979).
Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754–758 (1981).
Hsiang, M. W., Cole, R. D., Takeda, Y. & Echols, H. Nature 270, 275–277 (1977).
Sauer, R. T., Yocum, R. R., Doolittle, R. F., Lewis, M. & Pabo, C. O. Nature 298, 447–451 (1982).
McKay, D. B. & Steitz, T. A. Nature 290, 744–749 (1981).
Zubay, G. & Doty, P. J. molec. Biol. 1, 1–20 (1959).
Sung, M. T. & Dixon, G. H. Proc. natn. Acad. Sci U.S.A. 67, 1616–1623 (1970).
Adler, K. et al. Nature 237, 322–327 (1972).
Warrant, R. W. & Kim, S-H. Nature 271, 130–135 (1978).
Hol, W. G. J., van Duijnen, P. T. & Berendsen, H. J. C. Nature 273, 443–446 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pabo, C., Lewis, M. The operator-binding domain of λ repressor: structure and DNA recognition. Nature 298, 443–447 (1982). https://doi.org/10.1038/298443a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/298443a0
- Springer Nature Limited
This article is cited by
-
Vibrational spectrum and randomness of water at the interface of a protein–DNA complex
Theoretical Chemistry Accounts (2023)
-
Computational Simulation of Holin S105 in Membrane Bilayer and Its Dimerization Through a Helix-Turn-Helix Motif
The Journal of Membrane Biology (2021)
-
Structural study to analyze the DNA-binding properties of DsrC protein from the dsr operon of sulfur-oxidizing bacterium Allochromatium vinosum
Journal of Molecular Modeling (2019)
-
Crystal structure of the λ repressor and a model for pairwise cooperative operator binding
Nature (2008)
-
Cro, CAP and λ repressor led the way
Nature Structural Biology (1998)