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Specific differential expression of phospholipase A2 subtypes in rat cerebellum

  • Published:
Journal of Neurocytology

Abstract

Phospholipase A2 (PLA2) is a family of enzymes playing diverse roles in lipid signaling in neurons and glia cells. In this study, we examined the expression of subtypes of PLA2 in the cerebellum using immunolabeling and in situ hybridization methods. Two Ca2+-dependent cytosolic subtypes (cPLA2α and cPLA2β), one Ca2+-independent cytosolic subtype (iPLA2), and two secretory subtypes (sPLA2IIA and sPLA2V) were detected in the cerebellum. cPLA2α is present in somata and dendrites of Purkinje cells, while sPLA2IIA is associated with the endoplasmic reticulum in perinuclear regions of Purkinje cell somata. iPLA2 is present in granule cells, stellate cells and also in the nucleus of Purkinje cells. In addition, cPLA2β is localized in granule cells, and sPLA2V in Bergmann glia cells. These results provide an important basis for identifying functional roles of PLA2s in the cerebellum.

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  1. RIKEN, Wako, Laboratory for Memory and Learning, Brain Science Institute, Saitama, 351-0198, Japan

    Yoshinori Shirai & Masao Ito

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  1. Yoshinori Shirai
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  2. Masao Ito
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Shirai, Y., Ito, M. Specific differential expression of phospholipase A2 subtypes in rat cerebellum. J Neurocytol 33, 297–307 (2004). https://doi.org/10.1023/B:NEUR.0000044191.83858.f7

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  • DOI: https://doi.org/10.1023/B:NEUR.0000044191.83858.f7

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