Abstract
From the culture medium of Ralstonia (formerly Burkholderia or Pseudomonas) pickettii DSM 6297 grown under iron-limited conditions an iron complexing compound (siderophore) could be isolated. The structure of the isolated polycarboxylate siderophore was determined by spectroscopic methods as S,S-rhizoferrin, the enantiomer of R,R-rhizoferrin produced by fungi (Zygomycetes). Transport experiments with radiolabelled iron using S,S- and R,R-rhizoferrin showd no differences in the bacterial Ralstonia strain, while transport of R,R-rhizoferrin was superior in the producing fungal Rhizopus strain, suggesting stereoselective recognition in the fungus.
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References
Budzikiewicz H. 1997 Siderophores from fluorescent pseudomonads. Z Naturforsch 52c, 713-720.
Budzikiewicz H, Münzinger M, Taraz K, Meyer J.-M. 1997 Schizokinen, the siderophore of the plant deleterious bacterium Ralstonia (Pseudomonas) solanacearum ATCC 11696. Z Naturforsch 52c, 496-503.
Busse J, Auling G. 1988 Polyamine pattern as a chemotaxonomic marker within theproteobacteria. System Appl Microbiol 11, 1-8.
Carrano CJ, Thieken A, Winkelmann G. 1996 Specificity and mechanism of rhizoferrin-mediated metal ion uptake. BioMetals 9, 185-189.
Cornelis P, Hohnadel D, Meyer JM. 1989 Evidence for different pyoverdine-mediated iron uptake systems among Pseudomonas aeruginosa strains. Infect Immun 57, 3491-3497
Drechsel H, Metzger J, Freund S, Jung G, Boelaert JR, Winkelmann G. 1991 Rhizoferrin — anovel siderophore from the fungus Rhizopus microsporus var. rhizopodiformis. BioMetals 4, 238-243.
Drechsel H, Jung G, Winkelmann G. 1992 Stereochemical characterization of rhizoferrin and identification of its dehydration products. BioMetals 5, 141-148.
Drechsel H, Tschierske M, Thieken A, Jung G, Zähner H, Winkelmann G. 1995 The carboxylate type siderophore rhizoferrin and its analogs produced by directed fermentation. J Industrial Microbiol 14, 105-112.
Drechsel H, Winkelmann G. 1997 Iron chelation and siderophores. In: Winkelmann G, Carrano CJ, eds. Transition Metals in Microbial Metabolism. Amsterdam: Harwood Academic Publishers; 1-49.
Fernandez C, Wilhelmi I, Andradas E, Gaspar C, Gomez J, Romero J, Mariano JA, Corral O, Rubio M, Fereres J. 1996 Nosocomial outbreak of Burkholderia pickettii infection due to a manufactured intraveneous product used in three hospitals. Clin Infect Diseas 22, 1092-1095.
Kühn S, Braun V, Köster W. 1996 Ferric rhizoferrin uptake in Morganella morganii: characterization of genes involved in the uptake of a polycarboxylate siderophore. J Bacteriol 178, 496-504.
Shenker M, Ghirlando R, Oliver I, Helman M, Hadar Y, Chen Y. 1995 Chemical structure and biological activity of a siderophore produced by Rhizopus arrhizus. Soil Sci Soc Am J 59, 837-843.
Schwyn B, Neilands JB. 1987 Universal chemical assay for detection and determination of siderophores. Anal Biochem 160, 47-56.
Thieken A, Winkelmann G. 1992 Rhizoferrin: A complexone type siderophore of the Mucorales and Entomophthorales (Zygomycetes). FEMS Microbiol Lett 94, 37-42.
Thieken A, Winkelmann G. 1993 A novel bioassay for the detection of siderophores containing keto-hydroxy bidentate ligands. FEMS Microbiol Lett 111, 281-286
Winkelmann G. 1979 Evidence for stereospecific uptake of iron chelates in fungi. FEBS Lett 97, 43-46.
Winkelmann G, Braun V. 1981 Stereoselective recognition of ferrichrome by fungi and bacteria. FEMS Microbiol Lett 11, 237-241.
Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y. 1995 Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol 39,897-904.
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Münzinger, M., Taraz, K., Budzikiewicz, H. et al. S, S-rhizoferrin (enantio-rhizoferrin) – a siderophore of Ralstonia (Pseudomonas) pickettii DSM 6297 – the optical antipode of R, R-rhizoferrin isolated from fungi. Biometals 12, 189–193 (1999). https://doi.org/10.1023/A:1009259118034
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DOI: https://doi.org/10.1023/A:1009259118034