Abstract
Bifidobacteria assimilated raffinose about 4-fold more effectively than other intestinal bacteria, and α-galactosidase was active in all strains of Bifidobacteria tested. The enzyme activity of Bifidobacterium breve grown on raffinose was highly and specifically increased. Its activity was 30-fold higher than that of B. breve grown on glucose. Melibiose was also effective for production of the enzyme. The enzyme was purified to homogeneity from B. breve. It is a homodimer with Mr of about 160 kDa, and its optimum pH for activity of 5.5–6.5. The enzyme showed strict substrate specificity for α-galactoside although it had slight activity for α-glucoside. It hydrolysed stachyose, melibiose (Km = 2 mM) and raffinose (Km = 0.7 mM).
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**ao, M., Tanaka, K., Qian, X. et al. High-yield production and characterization of α-galactosidase from Bifidobacterium breve grown on raffinose. Biotechnology Letters 22, 747–751 (2000). https://doi.org/10.1023/A:1005626228056
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DOI: https://doi.org/10.1023/A:1005626228056