Abstract
MALDI, while providing advantages such as the ability to do in-depth and repeated exploration of the sample, challenges the existing performance capabilities of Fourier transform mass spectrometry (FTMS). The challenge arises because MALDI-produced ions have high mass-to-charge ratios and uncertain kinetic-energy distributions. We demonstrate that a combination of a gated trap** event, a RF-only mode pressure focusing event, and an electrically compensated trap provides a compelling advantage in meeting these challenges. Removal of any of the above combination elements significantly degrades the detection performance of substance P from 850 K resolving power at 34.9 kHz and of melittin from 278 K resolving power at 16.5 kHz when using a 3-Tesla magnet-based spectrometer.
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Published online June 9, 2004
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Gooden, J.K., Rempel, D.L. & Gross, M.L. Evaluation of different combinations of gated trap**, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry. J Am Soc Mass Spectrom 15, 1109–1115 (2004). https://doi.org/10.1016/j.jasms.2004.05.006
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DOI: https://doi.org/10.1016/j.jasms.2004.05.006