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Inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity reaction by antidepressant drugs

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An Erratum to this article was published on 01 May 2017

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Abstract

Background

Contact hypersensitivity (CHS) induced by a topical application of hapten - 2,4-dinitrofluorobenzene (DNFB), is a T cytotoxic (Tc)1-cell-mediated antigen-specific type of skin inflammation. Recently, it has been shown that antidepressant drugs inhibit the T helper (Th)1-mediated CHS reaction induced by picryl chloride. The aim of present study was to establish the effect of two-week desipramine or fluoxetine administration on the CHS reaction induced by DNFB.

Methods

Balb/c (H-2d) male mice were divided into six groups: 1) vehicle-treated negative control group; 2) desipramine-treated negative control group; 3) fluoxetine-treated negative control group; 4) vehicle-treated DNFB group (positive control group); 5) desipramine-treated DNFB group; 6) fluoxetine-treated DNFB group. T lymphocytes proliferation was determined by incorporation of [3H]-thymidine to DNA of concanavalin A stimulated cells. ELISA test was used for estimation of cytokines production.

Results

The antidepressants significantly suppressed the CHS reaction mediated by Tc1 cells: desipramine by 55% and fluoxetine by 54% compared to the positive control. Moreover, the antidepressants decreased the proliferative activity of splenocytes and the ability of splenocytes to produce interleukin (IL)-6 and interferon (IFN)-γ and increased IL-10 production by the lymph node (LN) cells of DNFB-treated mice.

Conclusion

The results of the present study show that the Tc1-dependent reactivity to DNFB is significantly suppressed by antidepressant drugs, which suggests their inhibitory effect on Tc1 mediated immunity.

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Change history

  • 30 December 2017

    The authors wish to note an incorrectly listed financial support grant. The National Science Centre grant 2011/01/B/NZ6/00300 was inadvertently listed. The authors regret this error. The correct Acknowledgements read as follows:

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Authors and Affiliations

  1. Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Science, Smętna 12, PL 31-343, Kraków, Poland

    Katarzyna Curzytek, Marta Kubera, Monika Majewska-Szczepanik, Weronika Duda, Monika Leśkiewicz, Agnieszka Basta-Kaim, Bogusława Budziszewska & Władysław Lasoń

  2. Department of Medical Biology, Jagiellonian University Medical College, Kopernika 12, PL 31-034, Kraków, Poland

    Marian Szczepanik, Katarzyna Marcińska & Włodzimierz Ptak

  3. Department of Toxicology, Jagiellonian University Medical College, Medyczna 9, PL 30-688, Kraków, Poland

    Bogusława Budziszewska

  4. Institute of Public Health, Jagiellonian University Medical College, Grzegórzecka 20, PL 31-531, Kraków, Poland

    Władysław Lasoń

  5. Maes Clinics & TRIA, 998 Rimklongsamsen Road, Bangkok, 10310, Thailand

    Michael Maes

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  1. Katarzyna Curzytek
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Correspondence to Marta Kubera.

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Curzytek, K., Kubera, M., Majewska-Szczepanik, M. et al. Inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity reaction by antidepressant drugs. Pharmacol. Rep 65, 1237–1246 (2013). https://doi.org/10.1016/S1734-1140(13)71481-6

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  • DOI: https://doi.org/10.1016/S1734-1140(13)71481-6

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