Abstract
The msh homeobox 1 (Msx1) and msh homeobox 2 (Msx2) coordinate in myoblast differentiation and also contribute to muscle defects if altered during development. Deciphering the downstream signaling networks of Msx1 and Msx2 in myoblast differentiation will help us to understand the molecular events that contribute to muscle defects. Here, the proteomics characteristics in Msx1- and Msx2-mediated myoblast differentiation was evaluated using isobaric tags for the relative and absolute quantification labeling technique (iTRAQ). The downstream regulatory proteins of Msx1- and Msx2-mediated differentiation were identified. Bioinformatics analysis revealed that these proteins were primarily associated with xenobiotic metabolism by cytochrome P450, fatty acid degradation, glycolysis/gluconeogenesis, arginine and proline metabolism, and apoptosis. In addition, our data show Acta1 was probably a core of the downstream regulatory networks of Msx1 and Msx2 in myoblast differentiation.
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Data are available via ProteomeXchange with identifier PXD026944.
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This work was supported by the grant from the National Natural Science Foundation Grant of China to **gqiang Wang (31972885).
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GZ: methodology, investigation, formal analysis, visualization, data curation, and original draft. SM: methodology and resources. MY: methodology, and data curation. YY: conceptualization, project administration, and reviewing and editing of the paper.
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43657_2022_49_MOESM1_ESM.tif
Fig. S1 RT-qPCR assay to valuate Acta1 expression in Msx1/2 over-expressed myoblast cells. Values are the means ± SD. ***p < 0.001 from unpaired student t tests. (TIF 153 kb)
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Zhou, G., Ma, S., Yang, M. et al. Global Quantitative Proteomics Analysis Reveals the Downstream Signaling Networks of Msx1 and Msx2 in Myoblast Differentiation. Phenomics 2, 201–210 (2022). https://doi.org/10.1007/s43657-022-00049-y
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DOI: https://doi.org/10.1007/s43657-022-00049-y